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Image Search Results
Journal: Cell Death & Disease
Article Title: Colorectal cancer patients-derived immunity-organoid platform unveils cancer-specific tissue markers associated with immunotherapy resistance
doi: 10.1038/s41419-024-07266-5
Figure Lengend Snippet: A Immunohistochemical staining of CD3, CD4, CD8, CD68 and Ki-67 in CRC tissues showed the presence of T-cells populations (CD4 + , CD8 + ) and macrophages (CD68 + ) in MSI and MSS patients. Ki-67 was used as a control proliferation marker of tumor cells; ( B ) Quantification of positive cells/area was performed with QuPath Software. Significance shown refers to Wilcoxon test p -values *< 0.05, **< 0.01, ***< 0.001.
Article Snippet: T-cells and Myeloid Derived Suppressor Cells (MDSCs) were isolated from patient peripheral blood mononuclear cells (PBMCs) by CD3 + immunomagnetic negative selection (#17951, StemCell Technologies) and CD33 + immunomagnetic positive selection (#17876, StemCell Technologies) following positive selection with
Techniques: Immunohistochemical staining, Staining, Control, Marker, Software
Journal: EBioMedicine
Article Title: Radiotherapy Upregulates Programmed Death Ligand-1 through the Pathways Downstream of Epidermal Growth Factor Receptor in Glioma
doi: 10.1016/j.ebiom.2018.01.027
Figure Lengend Snippet: The effect of PD-L1 blockade on the suppressing ability of tumor cells for CD8 + T cell proliferation. Cell lines (U87 and U251) were treated with RT (5 Gy), PD-L1 Ab (20 μg/mL), RT + PD-L1 Ab, AG490 (10 μM), RT + AG490, or RT + AG490 + PD-L1 Ab, and then co-cultured with CD8 + T cells. At 48 h, CD8 + T cell proliferation was evaluated by FCM. Non-specific Stimulation: anti-CD3/CD28 beads. Representative images and quantitative data are shown. Each column is shown as the means of three separate experiments; bars, SD. Student's t -test; *** P < 0.001. ANOVA; ns, nonsignificant.
Article Snippet:
Techniques: Cell Culture
Journal: EBioMedicine
Article Title: Radiotherapy Upregulates Programmed Death Ligand-1 through the Pathways Downstream of Epidermal Growth Factor Receptor in Glioma
doi: 10.1016/j.ebiom.2018.01.027
Figure Lengend Snippet: The effect of PD-L1 blockade on the cytotoxicity of CD8 + T cells against glioma cells. Cell lines (U87 and U251) were treated with RT (5 Gy), PD-L1 Ab (20 μg/mL), RT + PD-L1 Ab, AG490 (10 μM), RT + AG490, or RT + AG490 + PD-L1 Ab, and then co-cultured with CD8 + T cells. At 48 h, tumor cell apoptosis was evaluated by FCM. Representative images and quantitative data are shown. Each column is shown as the means of three separate experiments; bars, SD. Student's t-test; *** P < 0.001. ANOVA; ns, nonsignificant.
Article Snippet:
Techniques: Cell Culture